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Team:Groningen/Brainstorm/Glucose Sensing
From 2009.igem.org
Originally the Rainbow bacteria we now no longer plan to use the Cre/Lox system, but rather focus on glucose sensing. This choice was made because we have no good way to control the cre/lox recombination the way we would need for glucose sensing.
The idea now is to create a bacterium that senses the outside glucose concentration and produces an inversely proportional response.
Requirements
We use the [http://en.wikipedia.org/wiki/MoSCoW_Method MoSCoW prioritization method]:
- Must have this:
- Response should be inversely proportional to the outside glucose concentration.
- It should be measurable on-line (visually for example).
- Should have this if at all possible:
- Response should be a gradient (not just on/off).
- Delay in the response should not exceed roughly 5-10 minutes.
- (Model) parameters should be known or obtainable.
- Could have this if it does not affect anything else:
- Response should reasonably accurately follow the concentration (after a delay).
- Won't have this now but would like in the future:
- Expand to other metabolisms.
- Produce something more useful than a colour.
Design
Our bacterium would have the following components:
- The PTS system, which is already present in ... Which organisms?
- A promoter that hooks into the PTS system. Can be either:
- Constitutively active, but repressed by ccpa(?).
- Activated by ccpa(?).
- Product behind promoter as response.
- Degradation-tag(?) for product to increase response time.
