Team:Paris/Protocols

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Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.

iGEM paris 2009 protocols link :

our protocol:

• Protocol to make competent bacteria
• Protocole PCR quick load Taq mix
• [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method]

iGEM paris 2008 protocols link :

Click here

content :

• Electrophoresis
• Concentration of the Miniprep or the Midiprep
• Amplification of promoters.
• PCR Screening
• Protocol to make competent bacteria

iGEM paris 2007 protocols link :

[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]

content :

• Growing_bacteria_in_liquid_medium
• Preparing growth media
  • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
• Chemical transformation
• Glycerol Stock
• Recombineering/Lambda red-mediated gene replacement
• Miniprep
• Fluorescent single cells visualisation
• Digestion reactions

Protocols suggested