From 2009.igem.org
Wet
GVP Cluster
Over Night Cultures
Over night cultures in 4mL LB-amp100 or LB-kan50 medium were prepared from the following glycerol stocks:
- → E.coli TOP10 pSB3K3-high const. promoter (kan.)
- → E.coli TOP10 pSB3K3-med. const. promoter (kan.)
- → E.coli TOP10 pSB3K3-low const. promoter (kan.)
- → E.coli TOP10 pSB1AC3-high const. promoter (amp.)
- → E.coli TOP10 pSB1AC3-med. const. promoter (amp.)
- → E.coli TOP10 pSB1AC3-low const. promoter (amp.)
and put in the 37°C waterbath at 200 rpm.
Transporters
DONE
GlpF
→ pick colonies and use for overnight culture. (2 colonies in 6 mL LB-amp medium)
Metal Accumulation
TODO
MBP
- Plasmid isolation (cells can be found in the freezer Jolanda)
- PCR of MBP
-
12,5 uL
| Phusion 2x MM
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1 uL
| MBP Fw
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1 uL
| MBP rev
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0.5uL
| MBP plasmid
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- Restriction digest of pET-29a + SmtA and pGEX-3 + SmtA-GST
- Digest 500ng pET-29a + SmtA and pGEX-3 + SmtA-GST with AvaI(FD) in a total volume of 20uL.
- Incubate 30min @ 37;deg
- Run 10uL + 5uL loading dye on an 1% Agarose gel
2=1kb marker
4=SmtA-GST nr1/AvaI
5=SmtA-GST nr2/AvaI
6=SmtA-GST nr3/AvaI
8=SmtA nr1/AvaI
9=SmtA nr2/AvaI
Vectors
Dry
We (Jasper and Klaas) worked on deriving a method for estimating K and Vmax values based on combined import/export measurements. So far the application of this method on figure 3A of Kostal2004 has only yielded nonsensical results (K and V both negative and K (close to) zero if they are constrained to be positive).
we used the data of the plasmid, in the e. coli we used the chromosal ArsR, to see the difrence between the genes I have the data alignment with clustalW
April
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M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
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May
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M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_May_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_May_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
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June
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M | T | W | T | F | S | S
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_June_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
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[http://2009.igem.org/Team:Groningen/Notebook/8_June_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
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[http://2009.igem.org/Team:Groningen/Notebook/15_June_2009 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_June_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_June_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_June_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_June_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_June_2009 21]
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[http://2009.igem.org/Team:Groningen/Notebook/22_June_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_June_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_June_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_June_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
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[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
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July
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Team:Groningen/Notebook/1_July_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_July_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
|
[http://2009.igem.org/Team:Groningen/Notebook/6_July_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_July_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_July_2009 8]
| [http://2009.igem.org/Team:Groningen/Notebook/9_July_2009 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
|
[http://2009.igem.org/Team:Groningen/Notebook/13_July_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_July_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_July_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
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[http://2009.igem.org/Team:Groningen/Notebook/20_July_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_July_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_July_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_July_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_July_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_July_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
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[http://2009.igem.org/Team:Groningen/Notebook/27_July_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_July_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_July_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_July_2009 30]
| [http://2009.igem.org/Team:Groningen/Notebook/31_July_2009 31]
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August
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Team:Groningen/Notebook/1_August_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
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[http://2009.igem.org/Team:Groningen/Notebook/3_August_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_August_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_August_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_August_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_August_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_August_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
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[http://2009.igem.org/Team:Groningen/Notebook/10_August_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_August_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_August_2009 12]
| [http://2009.igem.org/Team:Groningen/Notebook/13_August_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_August_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_August_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_August_2009 16]
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[http://2009.igem.org/Team:Groningen/Notebook/17_August_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_August_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_August_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_August_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_August_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_August_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_August_2009 23]
|
[http://2009.igem.org/Team:Groningen/Notebook/24_August_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_August_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_August_2009 26]
| [http://2009.igem.org/Team:Groningen/Notebook/27_August_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_August_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_August_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_August_2009 30]
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[http://2009.igem.org/Team:Groningen/Notebook/31_August_2009 31]
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September
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Team:Groningen/Notebook/1_September_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_September_2009 2]
| [http://2009.igem.org/Team:Groningen/Notebook/3_September_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_September_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_September_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_September_2009 6]
|
[http://2009.igem.org/Team:Groningen/Notebook/7_September_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_September_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_September_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_September_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_September_2009 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
|
[http://2009.igem.org/Team:Groningen/Notebook/14_September_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_September_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_September_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_September_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_September_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_September_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
|
[http://2009.igem.org/Team:Groningen/Notebook/21_September_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_September_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_September_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_September_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
|
[http://2009.igem.org/Team:Groningen/Notebook/28_September_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_September_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_September_2009 30]
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October
|
M | T | W | T | F | S | S
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|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_October_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
|
[http://2009.igem.org/Team:Groningen/Notebook/5_October_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_October_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
|
[http://2009.igem.org/Team:Groningen/Notebook/19_October_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
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|
November
|
M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_November_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_November_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
|
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|