Team:Minnesota/Notebook

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Standard Protocols We Used in the Wet Lab

These link to pdf files:

Bacteria Culture Protocols

Transformation of Chemically Competent Cells

Plasmid Prep from Cultures

DNA Quantification

Polymerase Chain Reaction (PCR)

Restriction Digest

Vector Dephosphorylation

DNA Fragment Ligation

DNA Purification

Sequencing



Our Google Calendar

This calendar contains a day-by-day catalog of what we did in the wet lab for our project and parts characterization. The calendar for computational work can be found below. Please click on each event to see a detailed description of what we did.



Notebook




June
MTWTFSS
            [http://2009.igem.org/wiki/index.php?title=Minnesota/1_June_2008&action=edit 1]
[http://2009.igem.org/wiki/index.php?title=Minnesota/2_June_2008&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Minnesota/3_June_2008&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Minnesota/4_June_2008&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_June_2008&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Minnesota/6_June_2008&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Minnesota/7_June_2008&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Minnesota/8_June_2008&action=edit 8]
[http://2009.igem.org/wiki/index.php?title=Minnesota/9_June_2008&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Minnesota/10_June_2008&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Minnesota/11_June_2008&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_June_2008&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_June_2008&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Minnesota/14_June_2008&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Minnesota/15_June_2008&action=edit 15]
[http://2009.igem.org/wiki/index.php?title=Minnesota/16_June_2008&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Minnesota/17_June_2008&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_June_2008&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_June_2008&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_June_2008&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Minnesota/21_June_2008&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Minnesota/22_June_2008&action=edit 22]
[http://2009.igem.org/wiki/index.php?title=Minnesota/23_June_2008&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Minnesota/24_June_2008&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_June_2008&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_June_2008&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_June_2008&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Minnesota/28_June_2008&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Minnesota/29_June_2008&action=edit 29]
[http://2009.igem.org/wiki/index.php?title=Minnesota/30_June_2008&action=edit 30]
July
MTWTFSS
  [http://2009.igem.org/wiki/index.php?title=Minnesota/1_July_2008&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Minnesota/2_July_2008&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Minnesota/3_July_2008&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Minnesota/4_July_2008&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_July_2008&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Minnesota/6_July_2008&action=edit 6]
[http://2009.igem.org/wiki/index.php?title=Minnesota/7_July_2008&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Minnesota/8_July_2008&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Minnesota/9_July_2008&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Minnesota/10_July_2008&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Minnesota/11_July_2008&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_July_2008&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_July_2008&action=edit 13]
[http://2009.igem.org/wiki/index.php?title=Minnesota/14_July_2008&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Minnesota/15_July_2008&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Minnesota/16_July_2008&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Minnesota/17_July_2008&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_July_2008&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_July_2008&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_July_2008&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=Minnesota/21_July_2008&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Minnesota/22_July_2008&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Minnesota/23_July_2008&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Minnesota/24_July_2008&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_July_2008&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_July_2008&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_July_2008&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=Minnesota/28_July_2008&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Minnesota/29_July_2008&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Minnesota/30_July_2008&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Minnesota/31_July_2008&action=edit 31]
August
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Minnesota/1_August_2008&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Minnesota/2_August_2008&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Minnesota/3_August_2008&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Minnesota/4_August_2008&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_August_2008&action=edit 5] [http://2009.igem.org/Minnesota/6_August_2008 6] [http://2009.igem.org/wiki/index.php?title=Minnesota/7_August_2008&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Minnesota/8_August_2008&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Minnesota/9_August_2008&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Minnesota/10_August_2008&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Minnesota/11_August_2008&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_August_2008&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_August_2008&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Minnesota/14_August_2008&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Minnesota/15_August_2008&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Minnesota/16_August_2008&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Minnesota/17_August_2008&action=edit 17]
[http://2009.igem.org/wiki/index.php?title=Minnesota/18_August_2008&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_August_2008&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_August_2008&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Minnesota/21_August_2008&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Minnesota/22_August_2008&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Minnesota/23_August_2008&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Minnesota/24_August_2008&action=edit 24]
[http://2009.igem.org/wiki/index.php?title=Minnesota/25_August_2008&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_August_2008&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_August_2008&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Minnesota/28_August_2008&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Minnesota/29_August_2008&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Minnesota/30_August_2008&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Minnesota/31_August_2008&action=edit 31]
September
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Minnesota/1_September_2008&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Minnesota/2_September_2008&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Minnesota/3_September_2008&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Minnesota/4_September_2008&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_September_2008&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Minnesota/6_September_2008&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Minnesota/7_September_2008&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Minnesota/8_September_2008&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Minnesota/9_September_2008&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Minnesota/10_September_2008&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Minnesota/11_September_2008&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_September_2008&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Minnesota/13_September_2008&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Minnesota/14_September_2008&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Minnesota/15_September_2008&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Minnesota/16_September_2008&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Minnesota/17_September_2008&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_September_2008&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_September_2008&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Minnesota/20_September_2008&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Minnesota/21_September_2008&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Minnesota/22_September_2008&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Minnesota/23_September_2008&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Minnesota/24_September_2008&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_September_2008&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_September_2008&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Minnesota/27_September_2008&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Minnesota/28_September_2008&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Minnesota/29_September_2008&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Minnesota/30_September_2008&action=edit 30]
October
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Minnesota/1_October_2008&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Minnesota/2_October_2008&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Minnesota/3_October_2008&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Minnesota/4_October_2008&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Minnesota/5_October_2008&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Minnesota/6_October_2008&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Minnesota/7_October_2008&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Minnesota/8_October_2008&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Minnesota/9_October_2008&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Minnesota/10_October_2008&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Minnesota/11_October_2008&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Minnesota/12_October_2008&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Minnesota/13_October_2008&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Minnesota/14_October_2008&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Minnesota/15_October_2008&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Minnesota/16_October_2008&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Minnesota/17_October_2008&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Minnesota/18_October_2008&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Minnesota/19_October_2008&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Minnesota/20_October_2008&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Minnesota/21_October_2008&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Minnesota/22_October_2008&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Minnesota/23_October_2008&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Minnesota/24_October_2008&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Minnesota/25_October_2008&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Minnesota/26_October_2008&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Minnesota/27_October_2008&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Minnesota/28_October_2008&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Minnesota/29_October_2008&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Minnesota/30_October_2008&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Minnesota/31_October_2008&action=edit 31]
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