Team:Paris/Protocols

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Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.

Summary

1. Microscopy

• Protocols suggested

2. Adapted Protocols

• DNA extraction
• Mini prep
• Gel/PCR Purification

3. Culture protocols

• Over Night
• Bacterial transformation
• Competent bacteria
  • CaCl₂ (iGEM Paris2007)
  • CaCl₂ (2nd Protocol)
  • RbCl
• Glycerol stock

4. Molecular biology

• PCR
• Gels
• Digestion
• Ligation

• Protocole PCR quick load Taq mix
• [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method]
• Adaptation of PureYield™ Plasmid Miniprep System

A.2. iGEM paris 2008 protocols link

Click here

content :

• Electrophoresis
• Concentration of the Miniprep or the Midiprep
• Amplification of promoters.
• PCR Screening
• Protocol to make competent bacteria

A.3. iGEM paris 2007 protocols link

[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]

content :

• Growing_bacteria_in_liquid_medium
• Preparing growth media
  • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
• Chemical transformation
• Glycerol Stock
• Recombineering/Lambda red-mediated gene replacement
• Miniprep
• Fluorescent single cells visualisation
• Digestion reactions