Team:Paris/Protocols

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Contents

Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.

Main - Microscope Protocol - Adapted Protocols - Culture protocols - Molecular Biology
Main

Summary

1. Microscopy

  • Protocols suggested
  • [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]

2. Adapted Protocols

3. Culture protocols

4. Molecular biology

  • PCR
  • Gels
  • Digestion
  • Ligation

old

  • [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method]

A.2. iGEM paris 2008 protocols link

Click here


content :

  • Electrophoresis
  • Concentration of the Miniprep or the Midiprep
  • Amplification of promoters.
  • PCR Screening
  • Protocol to make competent bacteria

A.3. iGEM paris 2007 protocols link

[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]


content :

  • Growing_bacteria_in_liquid_medium
  • Preparing growth media
    • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions

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