Team:KULeuven/Primers
From 2009.igem.org
Number | Name | Sequence | Length | Tm (°C) | Comments |
---|---|---|---|---|---|
2171 | BLR promoter large fw | CATCAT GAATTCGCGGCCGCTTCTAGAG TTT GAC AGG TTC GTC GTC | 46 | 52.4 | it has a catcat and prefix (not in Tm included) |
2172 | BLR promoter large rv | CTG CAG CGG CCG CTACTAGTA CCT CTG TTA AAA ATG TTA ATC AAT GTT AAG | 51 | 52.1 | it has a suffix (not in Tm included) |
2260 | BLR promoter exact fw | CAT CAT GAA TTC GCG GCC GCT TCT AGA GAT TGC AAA AAA TTA ATT TAT CAT TCT G | 55 | 48.6 | it has a catcat and prefix (not in Tm included) |
2261 | BLR promoter exact rv | CTG CAG CGG CCG CTA CTA GTA AAA AAT GTT AAT CAA TGT TAA GTG AAG | 48 | 49.6 | it has a suffix (not in Tm included) |
2235 | Vanilline receptor Vir A fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA ACG GAA GAT ATT CAC C | 46 | 67 | it has a prefix |
2238 | Vanilline receptor Vir A rev | CTG CAG CGG CCG CTA CTA GTA TTA TCA CGT CTT GAT TTT GGT TAG | 45 | 66.1 | it has a suffix |
2242 | Vanilline receptor Vir G fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA TTG TAC ATC CTT CAC GTG | 48 | 67.6 | it has a prefix |
2243 | Vanilline receptor Vir G rev | CTG CAG CGG CCG CTA CTA GTA TTA TCA GGC TGC TGC CAT CGT C | 40 | 69.2 | it has a suffix |
2244 | Vanilline receptor Rpo A fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA TGA TTC AGA AGA ACT GGC AG | 47 | 67.9 | it has a prefix |
2245 | Vanilline receptor Rpo A rev | CTG CAG CGG CCG CTA CTA GTA TTA TTA GTA TTG GTC TTC GTA ACG C | 46 | 66.5 | it has a suffix |
2246 | Vanilline receptor vir B promoter regio fwd | CAT CAT GAA TTC GCG GCC GCT TCT AGA GTT CGT TCT GAG GCC GAC CTG | 48 | 70.7 | it has a prefix |
2247 | Vanilline receptor vir B promoter regio rev | CTG CAG CGG CCG CTA CTA GTA TCT CCT TAG CTC GCA ACT AAC ACC G | 46 | 69.9 | it has a suffix |
2292 | Vanilline receptor mutagenese Rpo A fwd | GTC CTG CAC GAA TTT TCC TCC ATC CCC GGC | 30 | 62 | EcoR1 op positie 202 |
2293 | Vanilline receptor mutagenese Rpo A rev | GCC GGG GAT GGA GGA AAA TTC GTG CAG GAC | 30 | 62 | EcoR1 op positie 202 |
2294 | Vanilline receptor mutagenese Rpo A fwd | GAA ATC CGC ATG GAA TTT ACC GTC AAC AAC GGC | 33 | 64.1 | EcoR1 op positie 427 |
2295 | Vanilline receptor mutagenese Rpo A rev | GCC GTT GTT GAC GGT AAA TTC CAT GCG GAT TTC | 33 | 64.1 | EcoR1 op positie 427 |
2288 | Vanilline receptor mutagenese vir A fwd | GAC AGC GCC TCA CTG CAA CGC GAT GTA CTC C | 31 | 68 | PstI op positie 184 |
2289 | Vanilline receptor mutagenese vir A rev | GGA GTA CAT CGC GTT GCA GTG AGG CGC TGT C | 31 | 68 | PstI op positie 184 |
2290 | Vanilline receptor mutagenese vir A fwd | ATT GCG GAG ATG CTG CAA CGC GAG TGT TTG G | 31 | 67.2 | PstI op positie 718 |
2291 | Vanilline receptor mutagenese vir A rev | CCA AAC ACT CGC GTT GCA GCA TCT CCG CAA T | 31 | 67.2 | PstI op positie 718 |