Template:Team:KULeuven/12 August 2009/BlueLightReceptor

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  1. Gel electrophorese of BlP cut with EcoRI and SpeI
  2. Gel extraction of the Blp

Nanodrop results

Part concentration (ng/μl) 260/280 λ 260/230 λ
Blp 27 2,08
  1. PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
    • Annealing of 58C
  2. Innoculate liquid medium
    • Innoculation of Psb3k3-P1010 and J23101
  3. Ligation
Vector insert
+P1010 (BLP)
50ng -> 2,5 μl 5 ng -> 0,3 μl
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