Template:Team:KULeuven/13 August 2009/VanillinProduction

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Revision as of 12:41, 14 August 2009 by JochemDeen (Talk | contribs)
  1. Plated ComT (+RBS) from the registry
    • Growing overnight in 37C
  2. Plated Ech
    • From newly made liquid culture
  3. Miniprep of Ech and Sam8
    • Nanodrop results:
Part concentration (ng/μl) 260/280 λ 260/230 λ
Ech 72,7 1,94
Sam8 66,2 2,00
    <il> Restriction digest on Ech and Sam8</il> <il> Gelelectrophorese on Ech and Sam8</il> <il> New electrocompetent cells were used for the electroporation using the old ligations</il>
      • Lig A -> Sam5 + Sam8
      • Lig C -> Ech + fcs
      • Note: there was no spark
    2. Plating of the electroporated cells
      • 200 μl per plate twice
      • 600 μl left of A and C
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