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From 2009.igem.org

Contents 1 Project 1 1.1 Synthesis of the Therapeutic DNA 1.2 Synthesis of the Gene Therapy Production System 1.3 Synthesis of the Targeted Biobrick 1.4 Production of the targeted gene therapy vector 1.5 Functioning of the targeted gene therapy vector 2 Project 2 3 Project 1 and Project 2

Project 1

Synthesis of the Therapeutic DNA

In this part, we aims at constructing a molecular cloning vector with a cos site which enables it to be packaged into the gene therapy vector- in other words, to construct a cosmid. In order to detect the expression of the therapeutic DNA, we construct this cosmid based on the scanfold of Parts-J61031 encoding a RFP-expressing segment.

This cosmid mainly consists of the following segments:

1) origin of replication: we insert O gene and P gene from bacteriophage lambda into J61031, which are resoonsible for the late phase replication and package of the wild type circular bacteriophage lambda genome

2) cos site: necessary for the specific package of the Therapeutic DNA into the gene therapy vector.

3) RFP-expressing segment: originally integrated into the plamid of J61031.

cosmidcostruct

Synthesis of the Gene Therapy Production System

Synthesis of the Targeted Biobrick

Production of the targeted gene therapy vector

Functioning of the targeted gene therapy vector

Project 2

Project 1 and Project 2

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