Team:SDU-Denmark/Protocols/Restrictions

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Sadly, we had a lot of trouble with our restrictions, and this page will reflect that in including alot of different restriction protocols.

Protocol 1

  1. Pool plasmid and dry on vacuum centrifuge down to about 50uL
  2. Mix the following into one tube:
    1. 4uL Plasmid and RIP
    2. 5uL 10x Buffer
    3. 0,5uL BSA
    4. Fill with water to 47uL (37,5uL)
    5. 1,5uL Enzyme 1
    6. 1,5uL Enzyme 2
  3. Incubate for 2 hours on 37 degress C.
  4. Inactivate the enzymes at 80 degress C for 20 minutes.
  5. Place product on -20 degress C or continue immediately to ligation.
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