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Team:Nevada/Project
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Overall project
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Project Details
The goal of our project is to clone one or more genes from the cinnamaldehyde pathway into E. coli as a potential insecticide against mosquito larvae. The ultimate goal of this project would be to move one or more of these genes into Wolffia, a small aquatic plant to which mosquito larvae feed upon during the spring and summer months.
Why Mosquitoes?
It is estimated that every year over 700 million people are infected by diseases spread by mosquitoes, and over 2 million of them die as a result. Control of mosquito populations in a cost effective and environmentally safe way is one of many ways to help lower the casualties.
Goal 1
The initial goal of this project is to clone the Arabidopsis gene cinnamaldehyde CoA-reductase into an E. coli expression system and test for activity. Currently, we are planning to use an inducible promoter, a RBS, and double terminator from the standard registry of parts to create this construct.
The Experiments
1. Conduct a three way ligation with pBAD (or LacI), RBS and a chloromphenicol resistant plasmid backbone (recombinant 1). 2. Conduct a three way ligation with cinnamaldehyde CoA, double terminator and chloromphenicol resistant plasmid (recombinant 2). 3. Conduct a three way ligation with recombinant 1, 2 and a tetracycline resestant plasmid.
Goal 2
