Team:HKUST/Protocols/gel cut
From 2009.igem.org
6. Cutting bands on the gel
Purpose: To extract specific DNA fragment. Procedure: a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA. b.Cut the band with a clean razor blade. c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube. Tips: •EB staining is needed for higher resolution under UV light. Safety Tips: •Permission is needed from technician before doing this! Follow the safety instruction in the room. •When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat.