| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| MC4100 | / | strain of E.coli | | | | july 24
|
| 2260 | / | forward primer for | | 42,5µl | 20μM | aug 10
|
| 2261 | / | reverse primer for | | 45µl | 20μM | aug 10
|
| | pSB1A2 | GFP + rbs + terminator | Miniprepped | 100μl | 85,6ng/μl | july 29
|
| | pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | 20μl | | july 29
|
| | pSB1A2 | GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | 30μl | 29,3 ng/μl | aug 14
|
| | pSB3K3 | kanamycine plasmide backbone | taken from plates | 13μl | | aug 11
|
| | / | Exact blue light promotor | pcr product | 20μl | 123,3 ng/μl | aug 13
|
| | / | constitutive promotor | taken from plates | 13μl | | aug 11
|
| | / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12
|
| + | pSB1A3 | LigA: Exact blue light promotor ligated with E0240 in pSB1A2 | T4 DNA ligation | 18μl | | aug 13
|
| + | pSB1A3 | Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 | T4 DNA ligation | 18μl | | aug 14
|
| + | / | Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,7,9) | cut with EcoRI and PstI | 4 x 18μl | | aug 24
|
| + | pSB1A2 | LigA: Exact blue light promotor ligated with E0240 in pSB1A2 | Miniprep (4x) | 20μl | | aug 18
|
| | pSB1A3 | constitutive promotor | miniprep | 10μl | 141,2ng/μl | aug 14
|
| (A) | | constitutive promotor | restriction digest with EcoRI en SpeI | 20μl | 20,8ng/μl | aug 24
|
| (B) | | constitutive promotor | restriction digest with EcoRI en SpeI | 10μl | 12,8ng/μl | aug 24
|
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| pSB1A2 | ech + rbs | purified plasmid (oranje) | | 108,8 ng/µl | August 4
|
| pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid (oranje) | | 101,3 ng/µl | August 5
|
| pSB1A2 | COMT | purified plasmid (oranje) | | 122,9 ng/µl | August 5
|
| pSB1A2 | fcs + rbs | purified plasmid (fcs pl.) | 29 µl | 57,2 ng/µl | August 12
|
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 pl.) | 31 µl | 76,0 ng/µl | August 12
|
| pSB1A2 | ech + rbs | purified plasmid (ech B) | 42,8 µl | 96,7 ng/µl | August 19
|
| pSB1A2 | ech + rbs | purified plasmid (ech C) | 48 µl | 105,6 ng/µl | August 19
|
| pSB1A2 | fcs + rbs | purified plasmid (fcs B) | 43,6 µl | 113,4 ng/µl | August 19
|
| pSB1A2 | fcs + rbs | purified plasmid (fcs C) | 48 µl | 90,3 ng/µl | August 19
|
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 B) | 44 µl | 125,0 ng/µl | August 19
|
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 C) | 48 µl | 104,2 ng/µl | August 19
|
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 B) | 40,1 µl | 63,2 ng/µl | August 19
|
| pSB1A2 | sam8 + sam5 | ligation mix (SAMS) | | | August 24
|
| pSB1A2 | fcs + ech | ligation mix (EF) | | | August 24
|
| pSB1A2 | fcs + ech | purified ligation plasmid (EF I) | 48 µl | 53,9 ng/µl | August 4
|
| pSB1A2 | fcs + ech | purified ligation plasmid (EF II) | 42 µl | 39,4 ng/µl | August 4
|
| pSB1A2 | fcs + ech | purified ligation plasmid (EF III) | 39,9 µl | 57,1 ng/µl | August 4
|
| pSB1A2 | sam8 + sam5 | purified ligation plasmid (SAMS I) | 48 µl | 76,8 ng/µl | August 4
|
| pSB1A2 | sam8 + sam5 | purified ligation plasmid (SAMS II) | 35,3 | 83,4 ng/µl | August 4
|
| pSB1A2 | sam8 + sam5 | purified ligation plasmid (SAMS III) | 39,3 µl | 61,5 ng/µl | August 4
|
| pSB1A2 | sam8 + sam5 | purified ligation plasmid (SAMS II), not purified from gel | 5 µl | | August 31
|
| pSB1A2 | sam8 + sam5 | purified ligation plasmid (SAMS I), purified from gel | 25 µl | | August 31
|
| pSB1A2 | fcs + ech | purified ligation plasmid (EF II), purified from gel | 5 µl | | August 31
|
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| 2235 | / | forward primer for | | | 20μM | August 3
|
| 2238 | / | reverse primer for | | | 20μM | August 3
|
| 2235 | / | forward primer for | | | 100μM | August 3
|
| 2238 | / | reverse primer for | | | 100μM | August 3
|
| 2242 | / | forward primer for | | | 20μM | August 3
|
| 2243 | / | reverse primer for | | | 20μM | August 3
|
| 2242 | / | forward primer for | | | 100μM | August 3
|
| 2243 | / | reverse primer for | | | 100μM | August 3
|
| 2244 | / | forward primer for | | | 20μM | August 3
|
| 2245 | / | reverse primer for | | | 20μM | August 3
|
| 2244 | / | forward primer for | | | 100μM | August 3
|
| 2245 | / | reverse primer for | | | 100μM | August 3
|
| 2246 | / | forward primer for | | | 20μM | August 3
|
| 2247 | / | reverse primer for | | | 20μM | August 3
|
| 2246 | / | forward primer for | | | 100μM | August 3
|
| 2247 | / | reverse primer for | | | 100μM | August 3
|
| 2288 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2289 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2288 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2289 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2290 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2291 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2290 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2291 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2292 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2293 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2292 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2293 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2294 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2295 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2294 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2295 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
"
