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Template:Team:KULeuven/29 August 2009/VanillinProduction
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Revision as of 10:00, 31 August 2009 by JochemDeen (Talk | contribs)
- Split the restriction digest in 2 parts
- 1 Part was directly used for ligation using double the mass required(Sams II and Ef II)
| Part | DNA ng | volume µl |
|---|---|---|
| Sam5 | 100 | 0,8 |
| Sam8 | 126,4 | 2,7 |
| Ech | 100 | 1 |
| Fcs | 244 | 2,2 |
- The 2nd part was first put on gel electrophorese and purified before it was used for ligation
- Gel electrophorese of restriction on Sam, Sam8, fcs and ech
- Sam5 and Sam8 showed expected results
- fcs and ech was unexpected
- fcs showed only 1 result on gel, expected two
- ech showed 4 results in gel (all too short), expected one
- Sam5 and Sam8 were purified and ligated (Sams I)
| Part | DNA conc ng/µl | 260/280 |
|---|---|---|
| Sam5 | 6,6 | 1,98 |
| Sam8 | 4,1 | 2,88 |
- Ech and Fcs were cut again and put overnight (overweekend...) in 16C
