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Template:Team:KULeuven/31 August 2009/BlueLightReceptor
From 2009.igem.org
- a Miniprep and RD (with EcoRI and PstI) were performed on the four colonies that were ented on sunday.
- nanoprop results
- RD results on gel: the right signals were detected
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA (1) | 110,6 | 1,92 | |
| LigA (2) | 76,7 | 1,99 | |
| LigA (3) | 84,8 | 2,07 | |
| LigA (4) | 31,7 | 2,02 | |
| 104,3 | 2,10 |
- ligA was plated on LB medium. this will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from.
- electroporation of in competent cells. it was plated and put overnight in the incubator.
- in order to be able to grow vector , which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. this is strain DB3.1 from Ecoli. these were plated and put in the incubator overnight.
