Template:Team:KULeuven/25 August 2009/BlueLightReceptor
From 2009.igem.org
- miniprepping the liquid cultures with
Part | concentration (ng/μl) | 260/280 λ |
---|---|---|
(1) | 27,6 | 1,91 |
(2) | 47,7 | 2,08 |
- restriction digest of with EcoRI and PstI
- gel electroforesis and extraction of the RD of
--> failed. there was no signal at all
- new colonies were ented in liquid culture.
- ligation C performed: (A) +
Vector | insert |
+ | |
30ng -> 1,4μl | 40,8ng -> 2μl |