Team:Wash U/Calendar-Home/1 September 2009

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9/1 Minipreps

number notation concentration
A RBS30_cph8 168.0
B ompR_term_ompC_GFP 161.0
C ompR_term_ompC_pucB/A 188.0

Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI [br] Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP and ompR_term_ompC_pucB/A as the vector for prefix insertion- cut with EcoRI and XbaI.

The Results of this digest are:

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The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A