Team:UNICAMP-Brazil/Notebooks/August 15

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YeastGuard: RBS and CFP plasmid digestion

- Today we started the construction of our first biobrick. After quantificate the mini-prep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.

- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.

- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.

Raíssa