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IBB Pune/1 September 2009
From 2009.igem.org
Finally standardized the PCR for a part of the competence gene regulon. Com EF from Acinetobacter baylyi was successfully amplified and the post PCR cleanup was done.
A 16 hr TA ligation reaction set up today to ligate the amplicon into standard pGEMT and pCR2.1 vectors.
