Template:Team:KULeuven/14 August 2009/VanillinReceptor

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  1. G 2,3,1' on gel electrophoresis
    • 2 and 3 seem oke, 1000bp
    • 1' seems oke
    • 800bp fragment + 200bp TOPO)
  2. Inoculate 2 and 3 and grow on ....
  3. TOPO from W and Z and again from A
    • Note: if A fails again (with the iGEM protocol) then proceed with a new PCR purification.
  4. Mutagenesis 1 on position 202 of R was performed
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