Biobrick/ Ligation Name |
Description |
Creation Date |
N° usage |
Strain |
Vector |
Quality Control |
J23100(J100) replaced by "J23100 Jun4" |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23101(J101) replaced by "J23101 Jun4" |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23102(J102) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23103(J103) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23104(J104) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23105(J105) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23106(J106) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23110(J110) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23112(J112) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23113(J113) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23114(J114) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23116(J116) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23117(J117) |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23118(J118) replaced by "J23118 Jun4" |
constitutive promoter |
May 25 |
0 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
E0240(GFP3) |
GFP protein generator (RBS-GFP-TT) |
May 25 |
5 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (MIT) |
K131009(E2) |
colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) |
May 28 |
1 |
TOP10 |
pSB1AK3 |
sequence BAD in prefix,suffix,celB (MIT,BMR) |
K117000(celB) |
colE7 lysis gene |
May 28 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
K124017(lysis21) |
Phage Lysis Cassette |
May 28 |
0 |
TOP10 |
pSB1AK3 |
sequence BAD (MIT) |
B0015(TT) |
Double terminator |
May 28 |
3 |
TOP10 |
pSB1AK3 |
sequence ok (MIT) |
I732017 (RBS-lacZ) |
RBS-lacZ |
May 28 |
1 |
TOP10 |
pSB1A2 |
long part (MIT) |
B0030 |
RBS 0.6 |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT,BMR) |
B0031 |
RBS 0.07 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
B0032 |
RBS 0.3 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
B0033 |
RBS 0.01 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
B0034 |
RBS 1 |
Jun 4 |
0 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
P0440(P04) |
RBS-tetR-TT |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (MIT) |
R0040(Ptet) |
Ptet |
Jun 4 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
T9002 |
Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT |
Jun 4 |
2 |
TOP10 |
pSB1A3 |
sequence BAD (MIT) - sequence ok (BMR) |
J23100(J100 Jun4) |
constitutive promoter |
Jun 4 |
3 |
TOP10 |
J61002(Amp) |
sequence ok (MIT,BMR) |
J23101(J101 Jun4) |
constitutive promoter |
Jun 4 |
2 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
J23118(J118 Jun4) |
constitutive promoter |
Jun 4 |
3 |
TOP10 |
J61002(Amp) |
sequence ok (MIT) |
A1 |
J23100(Jun 4)-E0240 (Pcon-RBS-GFP-TT) |
Jun 11 |
3 |
TOP10 |
pSB1A2 |
length ok (PCR) |
A2 |
J23101(Jun 4)-E0240 (Pcon-RBS-GFP-TT) |
Jun 11 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) |
A3-1 |
I732017-B0015 (RBS-lacZ-TT) |
Jun 12 |
2 |
TOP10 |
pSB1AK3 |
length ok (digestion PstI) sequence ok -long part- (BMR) |
A4 |
P0440-R0040 (RBS-tetR-TT-Ptet) |
Jun 11 |
2 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
A5 |
A4-A3 (RBS-tetR-TT-Ptet-RBS-lacZ-TT) |
Jun 19 |
0 |
TOP10 |
pSB1AK3 |
sequence ok -long part- (BMR) |
A6 |
J23100(Jun 4)-A4 (Pcon-RBS-tetR-TT-Ptet) |
Jun 19 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
BOL1 |
J23118-B0034-C0012-B0015 (Pcon-RBS-lacI-TT) |
Jun 23 |
3 |
TOP10 |
pSB1A2 |
sequence ok (BMR) |
A7 |
J23118(Jun 4)-E0240 (Pcon-RBS-GFP-TT) |
Jun 25 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) |
R0011(Plac) |
Plac |
Jun 25 |
1 |
TOP10 |
pSB1A2 |
sequence ok (MIT) |
K112808(ENTERO) |
holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) |
Jun 25 |
1 |
TOP10 |
pSB1A2 |
sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010). |
A8 |
A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jun 29 |
1 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion E-P) but works |
A9 |
A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jun 29 |
1 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion E-P) but works |
A10 thrown away |
K117000-B0015 (celB-TT) |
Jun 30 |
2 |
TOP10 |
pSB1AK3 |
sequence BAD (BMR) |
A8pg |
A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jul 3 |
1 |
TOP10 |
pSB1A2 |
length ok (digestion E-P) |
A9pg |
A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Jul 3 |
1 |
TOP10 |
pSB1A2 |
length ok (digestion E-P) |
A11-1 to throw away! |
BOL1-R0011 (Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence MUTATED |
A11-3 to throw away! |
BOL1-R0011 (Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) |
A11-6 to throw away! |
BOL1-R0011 (Pcon-RBS-lacI-TT-Plac) |
Jul 10 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) |
F2620 to throw away! |
Ptet-RBS-luxR-TT-Plux
|
Jul 10 |
1 |
TOP10 |
pSB1A2 |
sequence BAD (MIT, BMR) |
A12-2 |
J23118(Jun 4)-A4
|
Jul 16 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
A12-3 |
J23118(Jun 4)-A4
|
Jul 16 |
1 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
A14-4 to throw away! |
A11-E0240
|
Jul 16 |
0 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR) |
A14pg to throw away! |
A11-E0240
|
Jul 16 |
1 |
TOP10 |
pSB1A2 |
NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR) |
K116002 (PnhaA+GFP) |
PnhaA-RBS-GFP-TT
|
Jul 29 |
0 |
? |
pSB1A2 |
sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter |
K112405 (Pcad) |
cadAB promoter
|
Jul 29 |
0 |
? |
K112951(Amp+Cm) |
sequence ok (BMR) |
P0412 |
RBS-lacI-TT
|
Jul 29 |
0 |
? |
pSB1A2 |
sequence ok (BMR) |
I746902 |
GFPmut3+his tag generator under Pbad
|
Jul 29 |
0 |
? |
pSB1AK3 |
sequence ok -long part- (BMR) |
I746903 |
GFPmut3+his tag generator under PT7
|
Jul 29 |
0 |
? |
pSB1AK3 |
sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same |
K101017 to throw away! |
MioC promoter
|
Jul 29 |
0 |
? |
pSB1A2 |
sequence BAD (BMR) |
F2620MIT1 |
Ptet-RBS-luxR-TT-Plux
|
Jul 29 |
1 |
? |
pSB1A2 |
sequence ok (BMR) |
F2620MIT2 |
Ptet-RBS-luxR-TT-Plux
|
Jul 29 |
0 |
? |
pSB1A2 |
sequence ok (BMR) |
MRGENE1 |
pdc |
Jul 17 |
0 |
TOP10 |
pMK-RQ (Kan) |
antibiotic ok length ok (digestion X-P) sequence ok (Mr Gene) |
MRGENE1-2 |
pdc |
Jul 17 |
0 |
TOP10 |
pMK-RQ (Kan) |
antibiotic ok sequence ok (Mr Gene) |
MRGENE1-3 |
pdc |
Jul 17 |
0 |
TOP10 |
pMK-RQ (Kan) |
antibiotic ok sequence ok (Mr Gene) |
MRGENE2 |
adhB |
Jul 17 |
0 |
TOP10 |
pMA (Amp) |
antibiotic ok length ok (digestion X-P) sequence ok (Mr Gene) |
MRGENE2-2 |
adhB |
Jul 17 |
0 |
TOP10 |
pMA (Amp) |
antibiotic ok sequence ok (Mr Gene) |
MRGENE2-3 |
adhB |
Jul 17 |
0 |
TOP10 |
pMA (Amp) |
antibiotic ok sequence ok (Mr Gene) |
B1-13 |
B0030-MRGENE1 (RBS-pdc) |
Jul 28 |
1 |
TOP10 |
pSB1A2 |
length ok (digestion E-P , digestion S-P and digestion E-S) sequence ok (BMR) |
B2-5 |
B0030-MRGENE2 (RBS-adhB) |
Jul 23 |
2 |
TOP10 |
pSB1A2 |
length ok (PCR and digestion S-P) sequence ok (BMR) |
B3-5 |
B1-B0015 (RBS-pdc-TT) |
Jul 31 |
1 |
TOP10 |
pSB1AK3 |
length ok+extra band (digestion XhoI-P and digestion X-P) sequence ok (BMR) |
B4-2 |
B2-B0015 (RBS-adhB-TT) |
Jul 31 |
1 |
TOP10 |
pSB1AK3 |
length ok (digestion XhoI-P) sequence ok (BMR) |
B5-3 |
B1-B4 (RBS-pdc-RBS-adhB-TT) |
Aug 12 |
1 |
TOP10 |
pSB1AK3 |
length ok+2 extra bands (digestion E-P) sequence ok (BMR) |
B6-3 |
B2-B3 (RBS-adhB-RBS-pdc-TT) |
Aug 12 |
1 |
TOP10 |
pSB1AK3 |
length ok+2 extra bands (digestion E-P) sequence ok (BMR) |
A11-2 to throw away! |
BOL1-R0011 (Pcon-RBS-lacI-TT-Plac) |
Aug 13 |
1 |
TOP10 |
pSB1A2 |
length ok (digestion S-P) sequence MUTATED (BMR) |
A11-3 |
BOL1-R0011 (Pcon-RBS-lacI-TT-Plac) |
Aug 13 |
0 |
TOP10 |
pSB1A2 |
length ok (digestion S-P) sequence ok (BMR) |
A15-1 thrown away by mistake... |
F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 14 |
0 |
TOP10 |
pSB1A2 |
length ok (digestion) sequence ok (BMR) |
A15-2 |
F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 14 |
1 |
TOP10 |
pSB1A2 |
length ok (digestion) sequence WRONG (BMR) |
A15-3 |
F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 14 |
0 |
TOP10 |
pSB1A2 |
length ok (digestion) sequence ok (BMR) |
B8-5 thrown away! |
A4-B6 (RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT) |
Aug 21 |
1 |
TOP10 |
pSB1AK3 |
length maybe ok () sequence WRONG (BMR) |
A14-3 to throw away! |
A11(-2 Aug13)-E0240 (Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT) |
Aug 21 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence of A11-2(Aug13) was MUTATED (BMR) |
A16-4 |
A12-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT) |
Aug 21 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence ok (BMR) |
A17-4 to throw away! |
R0011-E0240 (Plac-RBS-GFP-TT) |
Aug 28 |
0 |
TOP10 |
pSB1A2 |
length ok (PCR) sequence WRONG! ligation failed. |
A18-? |
A15(-2)-pSB3K3 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T) |
Aug 28 |
0 |
TOP10 |
pSB3K3 |
still to screen |