| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| MC4100 | / | strain of E.coli | | | | july 24
|
| 2260 | / | forward primer for | | 42,5µl | 20μM | aug 10
|
| 2261 | / | reverse primer for | | 45µl | 20μM | aug 10
|
| | | GFP + rbs + terminator | Miniprepped | 100μl | 85,6ng/μl | july 29
|
| | | GFP + rbs + terminator | restricted with EcoRI and XbaI | 20μl | | july 29
|
| | | GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | 30μl | 29,3 ng/μl | aug 14
|
| | | kanamycine plasmide backbone | taken from plates | 13μl | | aug 11
|
| | / | Exact blue light promotor | pcr product | 20μl | 123,3 ng/μl | aug 13
|
| | / | constitutive promotor | taken from plates | 13μl | | aug 11
|
| | / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12
|
| + | | LigA: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | | aug 13
|
| + | | Lig B: Exact blue light promotor ligated with in | T4 DNA ligation | 18μl | | aug 14
|
| + | / | Lig A: Exact blue light promotor ligated with in (1,3,7,9) | cut with EcoRI and PstI | 4 x 18μl | | aug 24
|
| + | | LigA: Exact blue light promotor ligated with in | Miniprep (4x) | 20μl | | aug 18
|
| | | constitutive promotor | miniprep | 10μl | 141,2ng/μl | aug 14
|
| (A) | | constitutive promotor | restriction digest with EcoRI en SpeI | 20μl | 20,8ng/μl | aug 24
|
| (B) | | constitutive promotor | restriction digest with EcoRI en SpeI | 10μl | 12,8ng/μl | aug 24
|
| + | | LigA (1): Exact blue light promotor ligated with in | Miniprep | 45μl | 110,6ng/μl | aug 31
|
| + | | LigA (2): Exact blue light promotor ligated with in | Miniprep | 43μl | 76,7ng/μl | aug 31
|
| + | | LigA (3): Exact blue light promotor ligated with in | Miniprep | 44μl | 84,8ng/μl | aug 31
|
| + | | LigA (4): Exact blue light promotor ligated with in | Miniprep | 34μl | 31,7ng/μl | aug 31
|
| | pSB1A2 | RBS + GFP + terminator | Miniprep | 45μl | 104,3ng/μl | aug 31
|
| (A) | pSB1A2 | promotor | Miniprep | 48μl | 238,9ng/μl | sept 2
|
| (B) | pSB1A2 | promotor | Miniprep | 48μl | 248,8ng/μl | sept 2
|
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| | fcs + rbs | purified plasmid (FCS 1) | 13 µl | 32.1 ng/µl | September 1
|
| | fcs + rbs | purified plasmid (FCS 2) | 28 µl | 67.2 ng/µl | September 1
|
| | fcs + rbs | purified plasmid (FCS 3) | 28 µl | 82.4 ng/µl | September 1
|
| | ech + rbs | purified plasmid (ECH 1) | 15 µl | 37.2 ng/µl | September 1
|
| | ech + rbs | purified plasmid (ECH 2) | 28 µl | 26.9 ng/µl | September 1
|
| | ech + rbs | purified plasmid (ECH 3) | 28 µl | 28.0 ng/µl | September 1
|
| | Sam5 + rbs | purified plasmid (SAM5 1) | 19.6 µl | 59.9 ng/µl | September 1
|
| | Sam5 + rbs | purified plasmid (SAM5 2) | 28 µl | 52.8 ng/µl | September 1
|
| | Sam5 + rbs | purified plasmid (SAM5 3) | 13 µl | 67.3 ng/µl | September 1
|
| | Sam8 + rbs | purified plasmid (SAM8 1) | 12 µl | 31.0 ng/µl | September 1
|
| | Sam8 + rbs | purified plasmid (SAM8 2) | 8 µl | 47.2 ng/µl | September 1
|
| | Sam8 + rbs | purified plasmid (SAM8 3) | 28 µl | 42.9 ng/µl | September 1
|
| | Terminator | Terminator, B0015 | ±20 µl | 181,4 | September 3
|
| | sam8 | SAM8 RD 3/9 | 28 µl | 8,2 ng/µl | September 3
|
| | sam5 | SAM5 RD 3/9 | 28 µl | 1,4 ng/µl | September 3
|
| | ech | ECH RD 3/9 | 23 µl | 10,1 ng/µl | September 3
|
| | fcs | FCS RD 3/9 | 4 µl | 5,2 ng/µl | September 3
|
| | sam8 | SAM8 PCR | µl | 361.6 ng/µl | September 3
|
| | sam5 | SAM5 PCR | µl | 323.0 ng/µl | September 3
|
| | ech | ECH PCR | µl | 236.5 ng/µl | September 3
|
| | fcs | FCS PCR | µl | 321.4 ng/µl | September 3
|
| | ech + fcs | Ligatie zonder terminator(EFS) | µl | ng/µl | September 3
|
| | ech + fcs | Ligatie met terminator(EFT) | µl | ng/µl | September 3
|
| | sam5 + sam8 | Ligatie zonder terminator(58S) | µl | ng/µl | September 4
|
| | sam5 + sam8 | Ligatie met terminator(58T) | µl | ng/µl | September 4
|
| | ech + fcs | Miniprepped Ligatie Jochem (EF1) | 48 µl | 115.5 ng/µl | September 7
|
| | ech + fcs | Miniprepped Ligatie Jochem (EF2) | 48 µl | 105.2 ng/µl | September 7
|
| | ech + fcs | Miniprepped Ligatie Jochem (EF3) | 48 µl | 95.1 ng/µl | September 7
|
| | ech + fcs | Miniprepped Ligatie Jochem (EF4) | 48 µl | 95.7 ng/µl | September 7
|
| | sam5 | SAM5 purified | 23 µl | 10.0 ng/µl | September 7
|
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| 2235 | / | forward primer for | | | 20μM | August 3
|
| 2238 | / | reverse primer for | | | 20μM | August 3
|
| 2235 | / | forward primer for | | | 100μM | August 3
|
| 2238 | / | reverse primer for | | | 100μM | August 3
|
| 2242 | / | forward primer for | | | 20μM | August 3
|
| 2243 | / | reverse primer for | | | 20μM | August 3
|
| 2242 | / | forward primer for | | | 100μM | August 3
|
| 2243 | / | reverse primer for | | | 100μM | August 3
|
| 2244 | / | forward primer for | | | 20μM | August 3
|
| 2245 | / | reverse primer for | | | 20μM | August 3
|
| 2244 | / | forward primer for | | | 100μM | August 3
|
| 2245 | / | reverse primer for | | | 100μM | August 3
|
| 2246 | / | forward primer for | | | 20μM | August 3
|
| 2247 | / | reverse primer for | | | 20μM | August 3
|
| 2246 | / | forward primer for | | | 100μM | August 3
|
| 2247 | / | reverse primer for | | | 100μM | August 3
|
| 2288 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2289 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2288 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2289 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2290 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2291 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2290 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2291 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2292 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2293 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2292 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2293 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2294 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2295 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2294 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2295 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
"
