Team:KULeuven/4 August 2009

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Project progress

Progress of parts

[edit] Blue Light Receptor

[edit] Vanillin Production

Liquid cultures were made out of the sam5, sam8 and COMT colonies grown overnight. They will be mini-prepped tomorrow to get the plasmid DNA out.

Plasmid DNA from the ech and fcs liquid cultures was isolated today. It yielded respectively 108,8 and 113,9 ng/µl.

[edit] Vanillin Receptor

  • The primers made for the directed mutagenesis on Friday were ordered
  • PCR was done with primers for virA, virG, promotorregion virB and RpoA with template DNA from Agrobacterium tumefaciens pTiA6NC/A1011 to isolate the genes

[edit] Key/Lock/Anti-Key

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