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Team:KULeuven/17 August 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
Liquid cultures have been made for the kanamycine resistant plasmid backbone and for the LigA (BLP+ in ).
Lig B has been re-ented on a new plate because too many cells had grown on the one we made on aug 14. We always used single kolonies to re-ent.
[edit] Vanillin Production
- SAMS (ligation sam5+sam8) and EF (ligation ech+fcs): electroporated ...
- and plated out
[edit] Vanillin Receptor
- A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out.
- Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain
- Mutagenesis 2 on R on position 427 was performed with PCR
