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Team:KULeuven/24 August 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
- Plates with LigA were put under blue light. The LEDs were put on their max capacity.
- Restriction digest with
- tubes (1,3,5,7,9) of LigA (BLP + ) cut with EcoRI en PstI
- promotor cut with EcoRI en SpeI (4x)
- Gel electrophoresis with the RD of LigA and followed by a gel extraction:
- Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
- Note: the samples of promotor were barely visible. Only 2 of the 4 samples were recovered by extraction.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA 1 | 7,9 | 2,16 | |
| LigA 3 | 10,8 | 1,88 | |
| LigA 7 | 4,8 | 2,49 | |
| LigA 9 | 8,0 | 3,02 | |
| (A) | 20,8 | 1,70 | |
| (B) | 12,8 | 2,57 |
4. Enting of liquid cultures with kanamycin and
[edit] Vanillin Production
- Electroporation competent cells with EF and SAMS
- Cells were plated out and grown overnight
[edit] Vanillin Receptor
- TOPO vector with insert W showed some colonies. PCR + electrophoresis were performed for control but a bad and unclear picture was made so the result was not that visible
- B and G vector were digested to perform gelextraction
- B and G were put on a gel ==> length of the fragments is OK
- Enting of R202 in fluid medium en tomorrow second mutation (427)
