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Team:KULeuven/4 September 2009
From 2009.igem.org
Project progress
Progress of parts
[edit] Blue Light Receptor
- Gel extraction performed on the signals from 3/09
- Nanodrop: 14,7 ng/µl of with a 260/280 of approx 2,12
- Ligation with and (ligX 1)
- A new restriction digest on and with EcoRI and SpeI was performed.
- Gel extraction of these new digests. was nanodropped with a concentration of 28 ng/µl. this was ligated with (ligX 2)
- Electroporation of
[edit] Vanillin Production
- The restriction digest from SAMS and the terminator was put on gel
- It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be sam5 or sam8
- Electroporation of the ligation EF
- Plated and put at 37°C
- Liquid cultures made from sam5, sam8, ech and fcs.
[edit] Vanillin Receptor
- gelextraction and nanodrop for puc and A. The digest was done well.
- mutagenesis was tried with colony pCR on position 428
