Template:Team:KULeuven/18 August 2009/VanillinReceptor

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  • PCR purification miniprep and nanodrop of R on mutation 2
  • Start ethanol precipitation (overnight ethanol + sodium acetate) to create a better result (more purified product) of product R with mutation 202
  • PCR of the fragments of TOPO (W and Z) was done to check if TOPO worked. Gelelectrophoresis showed nothing so TOPO cloning failed again for virA.