Template:Team:KULeuven/25 August 2009/BlueLightReceptor

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  • Miniprepping the liquid cultures with
Part concentration (ng/μl) 260/280 λ
(1) 27,6 1,91
(2) 47,7 2,08
  • Restriction digest of with EcoRI and PstI
  • Gel electrophoresis and extraction of the RD of

--> failed. There was no signal at all

  • New colonies were ented in liquid culture.
  • Ligation C performed: (A) +
Vector insert
+
30ng -> 1,4μl 40,8ng -> 2μl
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