Team:Kyoto/Cells in Cells/Notebook/week1
From 2009.igem.org
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Project abstract
abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract
Contents
Monday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |
Tuesday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |
Wednesday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |
Thursday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |
Friday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |
Retrieved from "http://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week1"