Recombineering refers to the strategic use of recombination in vivo in order to reach a defined goal. In the case of BioBytes, a method is required to target the final construct to insertion at a specific place on the E. coli chromosome.

To do this successfully, three components must be taken into account:

- There must be a system for targeting the construct to a specific site for insertion

- Activation of the recombination system must be under experimenter control

- It must be possible to select for and verify colonies in which the insertion was successful