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Team:Virginia Commonwealth
From 2009.igem.org
Revision as of 22:18, 17 September 2009 by GMcArthurIV (Talk | contribs)
Promoter design, characterization and consequences
| The generation of well-characterized genetic parts (e.g., promoters, enhancers, RNA aptazymes) is a prerequisite for the rational design and construction of reliable genetically-encoded devices and systems. However, most opensource parts (including those in the Registry) remain uncharacterized. We propose a standard analytical approach for the quantitative characterization of promoter performance using both mRNA and protein measurements. In an effort to elucidate promoter design principles, we have also designed and characterized new promoter and enhancer sequences. Our goal is to contribute to the advancement of fundamental synthetic biology by characterizing new and existing promoters and enhancers, which may serve as a model for other basic parts such as ribosome binding sites (RBSs) and transcriptional terminators. | |
