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Template:Team:KULeuven/14 September 2009/VanillinProduction
From 2009.igem.org
Revision as of 13:28, 19 September 2009 by JochemDeen (Talk | contribs)
- Miniprep of COMT
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| COMT1 1 | 63,5 | 1,98 | |
| COMT1 2 | 99,4 | 1,95 | |
| COMT1 3 | 84,5 | 1,91 | |
| COMT2 1 | 92,1 | 2,00 | |
| COMT2 2 | 80,4 | 1,93 | |
| COMT2 3 | 74,2 | 1,95 | |
| COMT3 1 | 92,0 | 2,03 | |
| COMT3 2 | 62,2 | 2,04 | |
| COMT3 3 | 70,5 | 2,04 | |
| COMT4 1 | 93,4 | 1,91 | |
| COMT4 2 | 104,4 | 1,96 | |
| COMT4 3 | 81,9 | 2,01 |
- Restriction with S/P, SAMS2 P (was already cut with E/X) and EF3 with E/S
- Another attempt at electroporating EFT using EFT ligation from last week
- Gel electroforesis of 5 μl from the restriction sample (30 μl)
- Nothing in EF lane (no DNA???)
- Promotor showed again two results of 900bp and 2k bp (wut is going on?)
- An attempt was made to seperate the restriction enzymes by PCR purification on the remaining restriction sample, however nanodrop showed very confusing results, conclusion: let's not try this again. Next time heat inactivation of restriction enzymes to make sure they are inactive.
