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Team:Chiba/Notebook/Calendar/17 September 2009
From 2009.igem.org
(16_September_2009 <|>18_September_2009)
Contents |
Mini Prep
10:30~
- I731012(1930bp) pSB1A2(2079bp)
- K091134(2048bp) pSB1AK3(3189bp)
- S03956(1041bp) pSB1A2(2079bp)
- K091118(1985bp) pSB1A2(2079bp)
Digestion Test
14:30~
Double Digestion
| volume (uL) | |
| DNA | 3 (300ng) |
| EcoRI | 0.20 (4U) |
| PstI | 0.20 (4U) |
| Buffer(10x) | 1 |
| BSA(10x) | 1 |
| dW | 4.6 |
| total | 10 |
Single Digestion
| volume (uL) | |
| DNA | 3 (300ng) |
| EcoRI | 0.20 (4U) |
| Buffer(10x) | 1 |
| dW | 5.8 |
| total | 10 |
Gel
agar(low electro-osmosis)---0.7%(W)---0.42 g
TAE---60 ml
Energization
135V 27min
Result
from left to right
marker 1 2 3 4(no treatment) marker 1 2 3 4(single digestion) marker 1 2 3 4(double digestion)
ALL:OK
Lux Mutants Sequence
18:30 Mini prep
21:00 PCR
| sample number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| Big dye v3.1 premix | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| 5x sequencing Buffer | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| LuxR 3 (Mutant number) | 1 | 1 | ||||||||||||||
| 4 | 1 | 1 | ||||||||||||||
| 6 | 1 | 1 | ||||||||||||||
| 7 | 1 | 1 | ||||||||||||||
| 9 | 1 | 1 | ||||||||||||||
| 10 | 1 | 1 | ||||||||||||||
| 11 | 1 | 1 | ||||||||||||||
| L1 | 1 | 1 | ||||||||||||||
| Primer(VF) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | ||||||||
| Primer(VR) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | ||||||||
| dH2O | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 | 3.3 |
| TOTAL | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
Las Check
22:00 preculture LB-Amp 3 mL
- K084007
- K084012
- T9002
- K091134
- I731012
Lab Notebook
