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(23_September_2009 <|>25_September_2009)

Examine limit of AHL generation

Yesterday's operation is here.

• Today's operation

14:00

We made plates from each of mixture and 10 mL of LB-Amp, Cm solution medium.

• Element of mixtures

Sample Namber	1	2	3	4	5	6	7	8
supernatant solution	5 mL	---	---	---	---	---	---	20 μL
1	---	5 mL	---	---	---	---	---	---
2	---	---	5 mL	---	---	---	---	---
3	---	---	---	5 mL	---	---	---	---
4	---	---	---	---	5 mL	---	---	---
5	---	---	---	---	---	5 mL	---	---
6	---	---	---	---	---	---	5 mL	---
LB-Amp,Cm	10 mL	10 mL	10 mL	10 mL	10 mL	10 mL	10 mL	9.8 mL
Total	10 mL	10 mL	10 mL	10 mL	10 mL	10 mL	10 mL	10 mL

Transformation(2)-1

• Plasmid

plux-GFP(Cm, p15A)

• Competent Cells

JW1262(pCIA3-LuxR(Amp))

JW1262(Null)

21:35

We transplanted these bacteria each plates.

pCIA3-LuxR and plux-GFP --> LB-Amp, Cm plate

plux-GFP only --> LB-Cm plate

21:50-

We cultured these plates.

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