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Team:Newcastle/Meetings/16 April 2009
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Contents |
Instructor Meeting 16 April 2009
Students: Jess, James, Hanny, Goksel Instructors:
Apologies: Craig, Matt, Jane, Arun
Agenda of the Meeting
- More about flowchart/table/modelling
- iGEM wiki changes
- Europe Workshop: Most likely May 30/31 in London, UK at Imperial College.
- Lethbridge - Bacteria over Batteries - bacterial battery that uses photosynthesis to generate electricity.
- EPF-Lausanne - Brainstorm of 10 ideas.
Action Points of the Meeting
- Need to show normal growth on diagram
- Model population dynamics
- Ask Nigel Robinson about Metallisation
- Make powerpoint more detailed (biobrick slides)
- Name promoters that we are going to use
- Put them in the right order
- Finding a homologue comK
Minutes of the Meeting
- Knock out comK, and replace with one from another organism
- Sigma D Motility, provides bi-stability
- Work in a mutant background for biofilm formation, if we use sinI/sinR stochastic switch. Knocking out biofilm 'genes'
- Use KinA
- Methylase, no GFP. Instead of DNA rearrangement?
- RNAs Restriction enzyme, instead of DNAase to prevent germination, but don't know how it will work
- recA mutants, can repair themselves
- Metallothiene incorporate with whole set of coat proteins of the spore
- GFP and RFP with smtA to see where it binds on the coat of the spore
- Knock out phosphotases rap. spo0E sporelation
- Natural switches instead of synthetic ones
- Streptococci Stochastisity Competence
- Sigma F (spore specific) to control DNA rearrangement
- Negative feedback to control smtA expression
- Control mntH using mntR, also controls mntABCD, lets in Fe
- Strong promoters - Sigma K, cotC, cotA - can be used for smtA expression
- Sigma D is expressed all the time, provides bi-stability
- May be able to use E-Coli biobricks for DNA rearrangement
