Team:Tsinghua/Experiment1

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Synthesis of the Genome of GenSniper

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.

pET28a pACYCDuet1

Top-Down Approach

GenSniper Production Evaluation