Team:Chiba/Notebook/Calendar/28 September 2009

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Gel electrophoresis

  • Sample

Vector(plux-GFP), V-YFP, mOrange, and sfGFP



13:12

We made gel for electrophoresis.

agar 0.14 g

TAE 20 mL


14:08-

Gel electrophoresis(135 V, 27 min)


14:40-

Ethyl bromideに浸ける


15:05

Took a picture


Prepare for new gel electrophoresis

19:15

We cultured plux-GFP with 50 mL of LB-Cm in 300 mL flask at 37 degrees Celsius.

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