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Team:Johns Hopkins-BAG/Protocols
From 2009.igem.org
Contents |
Protocols
The Ligase Chain Reaction Protocol
Introduction
The Ligase Chain Reaction Protocol (LCR) is designed to replace and improve the current Templateless Chain Reaction Protocol (TPCR). The LCR is thought to have many distinct advantages: 1.) Accuracy of BB sequence should be increased (theoretically). 2.) The actual construction of the BB should be considerably easier thanks to the overlapping feature of the oligonucleotides. 3.) Money should be saved throughout the entire workflow because the LCR only needs to be run once to produce workable results. In order to make a working protocol we must first subject the given protocol to stress tests to test for robustness. The second part of our experiments should be to test known working samples of the LCR trials in order to prove that the experimental procedure works correctly. (We did this because our oligonucleotides were built incorrectly) We used samples from Jennifer Tullman’s batch of 3L.3_23.A1 BB. The third part of our testing will be to assemble the failed BB’s from the Intersession 2009 work that Mary and I worked on.
