Team:UNIPV-Pavia/Methods Materials/Fluorescence

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Fluorescence and water dispensation

Differently from the measures of absorbance, we find out that the dispensation of water in the well causes an amplification of the fluorescence signal, both for bacterial coltures and simple liquid growing medium (in figure the results for a colture producing GFP).

This amplification seams to be non linear and characterized by a kind of saturation, if analised on a large scale of volumes.

You can find more informations about the experiment at LINK - Test n.9, 07/07/09.

Fluorescence (GFP) and water dispensation, bacterial colture

Fluorescence and diluitions in water

Fluorescence and volume

Fluorescence and diluitions in liquid growing medium

Fluorescence and evaporation