Team:Washington/Accomplishments

BBa_K215002 Is a BioBrick part that was made to work in conjunction with the Secretion System (BBa_K215107). When used it creates a fusion protein, that contains the prtB secretion signal recognized by the secretion system. It also fuses to the protein a nano-tag that is able to bind Streptavidin, TEV protease site (for isolating your protein from the fusion) and Histidine Epitote Tags for easy protein purification.

This part is composed of:

BBa_K215000 -Protein Expression Vector BBa_K215001 -Fusion protein sequence

This part is used in: BBa_K215010 -GFP fusion protein BBa_K215011 -BBa_K215090 (organic phosphotriesterase) fusion protein BBa_K215091

Current Status: Complete

Future directions:

- Make more universal, to work with all proteins

- Optimize secretion-tag and nano-tag for fusion proteins

BBa_K215107 Is a BioBrick version of the Type 1 secretion system of Erwinia Chrysanthemi. It secretes proteins that contain the prtB C-terminus Epitote tag. To create a fusion protein with the prtB tag see BBa_K215002.

This part is composed of: BBa_K215100

BBa_K215101

BBa_K215102

BBa_K215103

BBa_K215104

BBa_K215105

BBa_K215106

BBa_K215108

BBa_K215109

Current Status: In progress

Future directions:

- Optimize RBS's

- Move secretion sequence into new plasmid, not TetR

- Try variety of cell lines.

BBa_K215200

BBa_K215201

BBa_K215210

BBa_K215211

BBa_K215250

BBa_K215251

BBa_K215260

BBa_K215261

Current Status: In progress

Future Directions:

- Use new CDS display vector to display streptavidin

- Design monomeric protein to bind peptide sequence

- Utilize other proteins on CDS to test for activity of other proteins

Biobricks Submitted	Current Status Report	Future Directions

Quick overview of what was done and the status of everyting. Maybe 3 paragraphs (display, secretion, conclusions)