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From 2009.igem.org

Contents 1 Synthesis of the Genome of GenSniper 1.1 Bottom-Up Approach 1.2 Top-Down Approach 2 GenSniper Production Evaluation

Synthesis of the Genome of GenSniper

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET-28a and pACYCDuet-1.

We incorporate the segment from Nu1 to B on bacteriophage lambda genome into pET-28a while clone the left segment from C to FII (C will be further engineered) into pACYCDuet-1. Before cotranformation with therapeutic DNA, we firstly cotranform the two plasmids encoding the GenSniper genome into BL21 DE3 and evaluate their function.

Top-Down Approach

GenSniper Production Evaluation

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