Team:Alberta/Project/Primer Design
From 2009.igem.org
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Primer DesignTo assemble the minimal genome, essential genes are first PCR amplified from genomic DNA and cloned into universal plasmids described in the DNA assembly section. By including restriction ennzyme cut sites on the primers, PCR products can be digested and ligated directly into the plasmid. We've designed over 300 primers for essential genes. Using these primers, over 150 essential genes have already been amplified. |