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August/6 August 2009
From 2009.igem.org
Contents |
Morning Meeting
To do in lab
1. Transform & Selection
- Medium buildng
- LB
- LB Amp+
- LB Kan+
- Transformation
- About 16 kinds of parts
We transform these following 14 parts today.
[http://partsregistry.org/Part:BBa_B0015 B0015] Plate 1 23L [http://partsregistry.org/Part:BBa_S03878 S03878] Plate2 16M
[http://partsregistry.org/Part:BBa_C0179 C0179] Pate 2 8M C0070 Plate2 12H
[http://partsregistry.org/Part:BBa_F1610 F1610] Plate2 24G [http://partsregistry.org/Part:BBa_B0034 B0034] Plate1 2M
[http://partsregistry.org/Part:BBa_I0462 I0462] Plate1 8O C0078 Plate1 14D
C0077 Plate1 14A [http://partsregistry.org/Part:BBa_I1466 I1466] Plate1 23J
2. Breeding
3. Refinement
To bring
- Timer
- Pen
- Slipper
Design genetic circuits
We have thus far focused our attention on signaling molecules used in quorum sensing systems of bacteria. The ones we will be using are as shown below:
*Inducer ⇒（synthesize) Signal molecule ⇒ Receptor
* LuxI ⇒ 3OC6HSL ⇒ LuxR → positive regulation * LasI ⇒ AI-1(3OC12HSL) ⇒ LasR → positive regulation * CinI ⇒ 3OH,C14:1-HSl ⇒ CinR → positive regulation * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR → positive regulation * AgrB synthesizes AIP from AgrD ⇒ AIP diffuses and attaches to AgrC ⇒ AgrC phosphorylates
and activates AgrA which then promotes transcription at P2 and P3 promoters.
From now on,We have to do what I mention below
1.Investigate other cell-cell comunication systems (besides those used in quorum-sensing).
2.Check to ensure that system components are working properly.
3.Create new systems or implement additional cell functions.
4.Investigate the possibility and extent of cross talk between various signaling molecules.
reported by Tadasi Nakamura
