August/12 August 2009

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1.before Min prep
Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies)
             [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 1-14F]                      50<
             [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 1-14H]                      50<
             [http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 1-23J]                       100~
             [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 1-12C]                       100~
             [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118013 2-18F]                       10

inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)

2.Transformation
to get new plasmid

each 4uL(DNA) , 1-2M , 1-12C : 2uL
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 1-2M] Amp
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 2-8O]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C2006 1-21F]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 2-21F]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 1-12A]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 2-8I]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 1-12C]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 2-24G]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 1-16G]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 1-18L] Kan
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 2-11N]

'8/11sample  10uL
RBS+2-8M(LasR)
EpsE+Terminater
(after 65℃ , 10min)

3.LB plate

LB(500uL)+Amp(2.5mL)  *25
                  +Kan(0.5mL)  *25

4.Min prep
Result of Nanodrop concentration check

(sample No.) (concentration)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005 I179005] (1-15N)             19.0 ng/uL 
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K091101 K091101] (2-6P)               27.0 
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 J23100] (1-18C)              21.5 
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0070 C0070] (1-12H)               48.0
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015] (1-23L)                23.7
                                         19.4  
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I)                    9.3
 
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I15009 I15009] (1-20F)                 71.3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118013 K118013] (2-18F)               19.2
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 C0078] (1-14H)                 49.9
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 I1466] (1-23J)                   21.3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C)                  10.7
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 C0077] (1-14F)                  32

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