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August/9 October 2009
From 2009.igem.org
We collected sequence data from the automated sequencer and used sequence alignment software to check the sequences against those obtained from the Parts Registry. The results were as follows:
Senders: LuxI - mismatched LasI - OK RhlI - OK CinI - unconfirmed (DNA sample concentration too low?)
Receivers: LuxR - OK LasR - mismatched RhlR - OK CinR - unconfirmed (DNA sample concentration too low?)
The DNA for Cin signaling system will be transformed, amplified and purified to obtain at higher concentration and PCR sequencing repeated on them. As time is running out, for the final circuit we shall use parts sent from Chiba University to replace our faulty ones.
