Team:Johns Hopkins-BAG/A New Standard

Revision as of 17:44, 12 October 2009 by Jdicarlo (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

[http://baderlab.bme.jhu.edu:8888/moodle18/ Moodle] [http://baderlab.bme.jhu.edu/syntheticyeast/wiki/index.php/Main_Page Synthetic Yeast Wiki] Protocols References Acknowledgements Contact

Someething

The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly

This Building Block standard can be either:

A) an interchangeable or B) a non-interchangeable format, based on the desired use of the part.