Team:Todai-Tokyo/Notebook/bread

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Plan

Aim:Create yeast that makes sweet and low energy sugar from starch

Methods:

  1. Clone the glucoamylase gene from Saccharomycopsis fibuligera
  2. Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination

Replace gpd gene by mtlD

September

  • PCR of mtlD
  • TA cloning of mtlD
  • PCR of gpd1 promoter

October

  • PCR of Glu1
  • cut mtlD by XbaI and PstI
  • PCR of gpd1 promoter with ExTaq






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