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Team:Todai-Tokyo/Notebook/bread
From 2009.igem.org
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Plan
Aim:Create yeast that makes sweet and low energy sugar from starch
Methods:
- Clone the glucoamylase gene from Saccharomycopsis fibuligera
- Clone mtlD (mannitol synthase) from E.coli and insert it in the yeast chromosome by homologous recombination
- Replace gpd gene by mtlD
September
- PCR of mtlD
- TA cloning of mtlD
- PCR of gpd1 promoter
October
- PCR of Glu1
- cut mtlD by XbaI and PstI
- PCR of gpd1 promoter with ExTaq
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