Team:IPN-UNAM-Mexico/Notebook/August 10 2009

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August 10th, 2009

Calculus for Mix; plasmid linearization.

For 20 reactions.

Electrophoresis gel load:

• 3 μl of colorant
• 10 μl of digestion
• 3 μl of molecular weight marker

Load order.

Well     Plasmid            Well             Plasmid

2          16,2”               13            3.3
3          4:23 L2            14            22.1
4          15:3 L2            15            19.2*
5          18.2*               16            24.2”
6          2.3

Well                             Well
1          1kb 2 μl         12            2.3
2          16.2”             13            14.2
3          4:23 L2          14            3.3
4          15:3 L2          15            22.1
5          18.2              15            19.1*
6          15.13

Horizontal Gene Transfer (HGT).
Acording to the model proposed to Arturo, we made a search for the candidate bioparts to make their elusion and transformation, which got in the next way:

2 Constructions:

Construction 1:
Biopart BBa_K145201

J23116 + B0034 + C0040 + B0010 + B0012

Construction 2:
The first option for the construction 2 is made up from two devices: BBa_J01101 and Bba_I763011. The second one consists in asking Boston for the construction BBa_I763027. We will tell Arturo about this posibility.
First option:
Device 1.
B0040 + B0034 + C0051 + B0010 + B0012
Device 2.
B0051 + B0034 + J04031

LOCATION